Natalie K. Barker - 2014-2019
As the Assistant Director of Proteomics, Natalie is responsible for the general operation and maintenance of two Thermo Orbitrap Fusion Lumos mass spectrometers with DIONEX nano liquid chromatography systems. In this role, Natalie has collaborated with researchers throughout the UofA campus that are interested in performing quantitative proteomics, assist with experimental design, teach mass spectrometry based protocols, and finally help in any way possible to make sure users acquire the highest quality, manuscript-ready data. To this end, she is responsible for the continuous optimization and adaptation of mass spectrometry protocols for maximizing efficiency and increased workflow. Protocols that are performed in the Proteomics Laboratory include in solution digestion, fractionation by in gel digestion, phosphopeptide enrichment, and filter-aided sample prep.
As a Senior Research Specialist in Dr. Paul Langlais’ lab, Natalie’s work centers on discovering new CLASP2 protein-protein interactions via the utilization of immunoprecipitation-based MS interactome analyses with the overall goal of discovering new insulin-dependent biological pathways of CLASP2. Some of the laboratory techniques/skills that Natalie proficient in include but are not limited to gel electrophoresis, immunoprecipitations, Western blots, polymerase chain reaction (PCR), Bradford assays, MS sample preparation, development of plasmid constructs, and general cell culture techniques. Additionally, Natalie assists with the preparation of figures and text for manuscripts and grants, present data at research laboratory meetings, train new lab members, and ensure the general upkeep and maintenance of the laboratory environment.
James Krantz - 2014-2019
Since October of 2014, James have been a Senior Research Specialist in the laboratory of Dr. Paul Langlais. Throughout his time in this role, James main research goals have been to characterize the CLASP2 interactome in adipocytes and myotubes, characterize the interactomes of CLASP2 interacting proteins, and to further elucidate the functions of CLASP2 in insulin signal transduction. James perform a wide array of general laboratory biochemical, molecular biology, cell culture, and mass spectrometry based techniques. He is responsible for collaborating with researchers and technicians from other groups, ordering lab products/equipment, set up/organization of the laboratory environment, assisting with manuscript and grant preparation, participating in group lab meetings and journal club presentations, training new lab members, and lab/equipment maintenance.
James was awarded a B.S. degree in Biology from Indiana University in 2011. After his undergraduate studies, he moved to The Mayo Clinic in Scottsdale, AZ, where he worked for five years as a Research Technologist. At this institution, James held several different positions working in academic and clinical research roles that provided him with a wide range of skills. Techniques that James gained experience in include but are not limited to microscopy, histology, clinical research laboratory techniques, and the handling and manipulation of mice and rats for in vivo metabolic studies.
Mackenzie Roman - 2019-2021
In the Fall of 2019, Mackenzie came to us from the Gus Mounemnie Laboratory over in the Cellular & Molecular Medicine Department where she worked all during college and for a year or two after. Mack brought a lot of cell biology experience which was a huge asset, she was basically a walking R01 grant. Mack had a strong interest to transition to a PhD program and so she was only able to stay with us for less than two years, she's currently getting ready to start graduate school in Utah so good luck Mac!!!
post-doc / fellow
James L. Dillon, MD - 2018
James Dillon has a nice history with the Langlais Lab. While both at good ol' ASU, James did his Undergraduate Honors Thesis with Paul and then left for medical school at UA Phoenix as part of the inagural class. After graduating James spent about a year back again with Paul in 2018 helping out with all kinds of stuff. James left to be a fancy doctor. Lame.